The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively.
ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif.
Phosphorylation at both Thr-183 and Tyr-185 is required for full enzymatic activation.
In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (5,6).
Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene.
Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases.
Nlk is a nuclear protein with the ability to autophosphorylate.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of NLK. Species Crossreactivity: Human, mouse, rat
