84-2370-89　［取扱停止］NLE1 ( Notchless Protein Homolog 1, NLE1, HUSSY-07) 50ul　224555

特徴

• The Notch signaling pathway is an evolutionary conserved system that is involved in intracellular communication.
• Notch receptors play an important role in development and cell-fate decisions.
• Notchless is a loss-of-function mutant allele that encodes for protein NLE1 (notchless homolog 1).
• NLE1 is a 485 amino acid WD40-repeat protein that binds to the cytoplasmic domain of Notch, regulating its signaling activity in Drosophila melanogaster and in mice.
• Deletion of the NLE1 gene in mice during the early stages of development results in embryonic death, while gene deletion in the late stages of development leads to activation of a caspase-3-dependent apoptotic pathway.In plants, NLE1 is crucial for normal cellular growth and development.
• Underexpression during shoot proliferation causes pleiotropic defects such as delayed flowering and abnormal organ maturation.
• It may also play a role in 60S ribosomal subunit biogenesis in yeast.
• NLE1 contains eight WD40 domains and produces one isoform due to alternative splicing.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.