Nkx-2.5, which is also designated cardiac specific homeobox protein (Csx), is a homeodomain-containing nuclear transcription protein of the Nkx-2 gene family.
These transcriptional activators, which include thyroid transcription factor-1 (TTF-1), regulate the expression of tissue specific genes and are required for maintaining the differentiated phenotypes of various lineages.
Nkx-2.5 is a homolog to the tinman protein expressed in Drosophila, and is essential for normal cardiovascular development.
Expression of Nkx-2.5 during cardiomyogenesis is required for cardiac septation, in which a single atrium and ventricle are separated into four chambers.
During embryonic development, Nkx-2.5 is also expressed in the foregut, thyroid, spleen and stomach, while in the adult expression is predominantly restricted to the heart.
Mutations that disrupt Nkx-2.5 can result in atrial-septal defects, embryonic lethality and congenital heart disease.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human NKX2-5.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to full length human NKX2-5 (P52952).
