Substance P (SP) and neurokinin-A (NK-A) are members of the tachykinins, and they function as modulators of the immune and hematopoietic systems.
The tachykinins interact with each of three cloned neurokinin (NK) receptors (NK-1R, NK-2R, NK-3R), with SP and NK-A exhibiting binding preferences for NK-1R and NK-2R, respectively.
NK-4R shares close homology with NK-3R, and both have nearly identical pharmacologicl properties.
In the normal ileum and colon, NK-1R and NK-2R are localized to smooth muscle cells of the muscularis mucosae and propria and to a few inflammatory cells of the lamina propria.
NK-1R expression is also found in the muscular wall of submucosal blood vessels, enteric neurons and, to a lesser degree, in surface epithelial cells.
NK-3R is found in the spinal cord in both lamina X and lamina II.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NK-3R. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 404-450 of Human NK-3R.
