NIPP1 (nuclear inhibitor of protein phosphatase 1) is a putative transcription regulator that may be involved in pre-mRNA splicing and cell proliferation.
NIPP1 contains a nuclear signaling region named FHA (fork-head associated) domain.
The FHA domain has been associated with protein kinases and transcription factors.
The NIPP1 locus encodes for three different isoforms termed alpha, beta and y due to alternative splicing events.
The isoforms exhibit RNA binding activity and also act as phophatase inhibitors.
The y isoform is believed to be a magnesium dependent endoribonuclease that is responsible for cleaving RNA strands.
It is mainly found in B cells and T lymphocytes.
The alpha and beta isoforms are localized in the brain and kidney.
Inactivation of NIPP1 is accomplished by the phosphorylation of Ser 199 or Ser 204.
NIPP1 interacts with proteins CDc5L, SAP 155, MELK and EED.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NIPP1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 237-282 of Human NIPP1.
