The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH.
These proteins are all necessary for archaeal and bacterial nitrogen fixation.
The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer.
NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner.
NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NIFK. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 196-245 of Human NIFK.
