The NFAT (nuclear factor of activated T cells) family of transcription factors include the cytoplasmic NFAT transcription factors (NFATc1, NFATc2, NFATc3, NFATc4 and NFATc5) and nuclear NFAT (NFATn).
Although primary cytoplasmic proteins, the nuclear translocation and transcriptional activity of the NF-ATc family is essential to developmental, differentiation, and adaptation processes.
NFATc1 is present in uninduced cells and translocates to the nucleus upon calcium mobilization.
The phosphatase calcineurin promotes nuclear accumulation of NF-ATc.
PKA causes phosphorylation and cytoplasmic accumulation of NF-ATc1 in direct opposition to calcineurin by phosphorylating Ser-245, Ser-269, and Ser-294 in the conserved serine-proline repeat domain.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of NFATC1. Species Crossreactivity: Human, mouse, rat
