Neurofibromatosis type 1 (NF1), or von Reckinghausen neurofibromatosis, is one of the most common autosomal dominant disorders in humans.
Early linkage analysis mapped the NF1 gene to chromosome 17.
The predicted NF1 transcript encodes a 2818 amino acid protein designated NF1GRP.
By sequence analysis, similarity has been demonstrated within a small region of NF1GRP and members of the Ras GAP gene family.
Functionally, NF1GRP was shown by biochemical analysis involving RAS-GAP hydrolysis and functional complementation in yeast to further resemble GAP protein.
The NF1 protein is expressed at relatively constant levels in a broad range of cell lines and tissues including brain, lung, liver, kidney, spleen, muscle and colon.Although little is known regarding the function of NF1GRP, the homology with the catalytic domain of proteins with GTPase activity suggests that the NF1GRP may also interact in vivo with Ras proteins.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Neurofibromin. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1550-1600 of Human Neurofibromin.
