84-2368-73　［取扱停止］NEIL1 (DNA (apurinic or apyrimidinic site) Lyase Neil1, DNA Glycosylase/AP Lyase Neil 1, DNA Glycosylase/AP Lyase Neil1, Endonuclease 8 like 1, Endonuclease VIII, Endonuclease VIII like 1, FPG1, hFPG1, NEH 1, NEH1, NEI 1, Nei Endonuclease VIII like 1 (E. coli), Nei Endonuclease VIII like 1, Nei Homolog 1, Nei like 1, Nei like Protein 1, NEIL 1, Neil1) 50ul　224446

特徴

• NEIL1, NEIL2 and NEIL3, also known as Endonuclease VIII-like 1,2 and 3 or DNA-(apurinic or apyrimidinic site) lyase NEIL 1, 2 and 3, are nuclear proteins involved in the repair of DNA damaged by oxidation.
• The NEIL proteins belong to the FPG familiy of proteins.
• They act as DNA glycosylases that can recognize and remove damaged bases, leaving an abasic site.
• NEIL3, however, lacks the proline residue at the N-terminus which acts as the active site residue found in NEIL1 and NEIL2.
• NEIL1 is a ubiquitiously expressed protein that is up-regulated during S-phase.
• NEIL2 is expressed primarily in testis, heart, skeletal muscle, placenta, brain, kidney and liver while NEIL3 is detected primarily in thymus and testis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.