84-2368-64　［取扱停止］Neddylin (NEDD8, Neddylin, Neural Precursor Cell expressed Developmentally down-Regulated Protein 8, NEDD-8, Ubiquitin-like Protein Nedd8, NEDD8) 100ul　224441

特徴

• NEDD8 is a ubiquitin (Ub)-like molecule that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation.
• NEDD8 and the corresponding yeast homolog RUB1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters.
• Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, UBC12.
• NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein von Hippel Lindau (VHL).
• NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle.
• In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.