C23 (nucleolin, NCL) is a eukaryotic nucleolar phosphoprotein that influences synthesis and maturation of ribosomes.
C23 localizes to dense fibrillar regions of the nucleolus.
It contains four RNA binding domains that interact with pre-rRNA during synthesis.
C23 can influence RNA processing, ribosomal gene transcription and nucleolar targeting of ribosomal components.
It is known to associate with a variety of proteins, including the nucleolar protein B23.
Phosphorylation by Cdc2 and casein kinase II causes translocation of C23 from the nucleolus to the cytoplasm.
Mitotic phosphorylated forms of Bcl-2 are present in nuclear structures in prophase Hela cells together with C23 and Ki-67.
Retinoic acid-induced apoptosis leads to C23 down-regulation and Bcl-2 mRNA instability.
C23 binds the human telomerase reverse transcriptase subunit (TERT) through interactions with its RNA binding domain 4 and carboxyl-terminal RGG domain, and this interaction is critical for the nucleolar localization of human TERT.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of NCL. Species Crossreactivity: Human, mouse, rat
