The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKalpha) and NCK2 (NCKbeta), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins.
Specifically, overexpression of NCK1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF.
Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis.
The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator.
The NCK protein thus mediates the interaction between PAK and RAC.
The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.
Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Nck-2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 341-390 of Human Nck-2.
