Pre-B cell-enhancing factor (PBEF), also designated nicotinamide phosphoribosyltransferase (Nampt) or visfatin, belongs to the NAPRTase family of proteins.
PBEF may be involved in enhancing the effect of IL-7 and SCF on the formation of early B-lineage precursor colonies.
It is involved in the catalysis of nicotinamide with 5-phosphoribosyl-1-pyrophosphate, yielding nicotinamide mononucleotide, which is important in NAD biosynthesis.
This is a rate limiting step in the NAD biosynthesis pathway.
Highly enriched in the visceral fat of both human and mouse, PBEF expression levels in plasma increase during the development of obesity.
PBEF is a cytoplasmic protein expressed primarily in bone marrow, muscle and liver tissue, but it can also be detected in placenta, lung, kidney and heart tissue.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of NAMPT. Species Crossreactivity: Human, mouse, rat
