Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin–protein ligase (E3), Nedd4.
Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity.
We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates.
In particular, we found that N4BP1 and p73alpha, a target of Itch-mediated ubiquitin/proteasome proteolysis, share the same binding site.
By competing with p73alpha for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73alpha and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.
Applications:Suitable for use in Western Blot andImmunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 15mM sodium azide.
Specificity：Recognizes endogenous levels of human N4BP1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 391-440 of human N4BP1 (O75113).
