coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches.
Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair.
MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage.
Multiple N-terminal splice variants of MYH exist in mammalian cells.
Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival.
Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas.
MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MYH. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 142-190 of Human MYH.
