Mucin 6 (also designated MUC 6 and gastricmucin) is a large glycoprotein that plays amajor role in the protection of the gastrointestinal tract.
MUC6 carries GlcNAcalpha1→4Galbeta→R structures, indicating thatalpha1, 4-N-acetylglucosaminyltransferase isimportant to the formation of the mucous glycoproteinsin vivo.
Mucin 6 expression is highest in the stomach and gall bladder, with lower expression in the terminal ileumand rightcolon.
In gastriccancer, Mucin 6 has an altered expression.
In normalstomach, Mucin 6 apomucin is associated with Lewistype 2; Mucin 6 is also expressed in gastric metaplasia, duodenum and pancreas.
Mucin 6 is a secretory mucin, located in the deepermucosal folds of human gall bladder, and its expression is altered with increasing degrees of inflammation.
Mucin 6mRNA is expressed transientlyin the nephrogeniczone of the kidneyin the early mid-trimester of development, and Mucin 6 glycoprotein is expressed in the epithelium of ureteric buds and collecting ducts, but absent fromadultkidney.
Proliferating bile ductularcells express Mucin 6 apomucin in diseased liver, especially in chronicviral hepatitiswith active necroinflammation,suggesting that this secreted mucin acts as a cytoprotective agent and represents a phenotype of reactive biliary epithelium in chronicviral hepatitis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：lSupplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human MUC6 at ~260kD. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in human MUC6
