MTDH (metastasis adhesion protein), also known as astrocyte elevated gene-1 protein (AEG1) or lysine-rich CEACAM1 co-isolated protein (LYRIC), is a 582 amino acid single-pass membrane protein.
Overexpressed in various cancers, MTDH activates the transcription factor NFκB and promotes anchorage-independent growth of immortalized astrocytes and melanocytes, which are key components of tumor growth.
MTDH is also thought to enhance the seeding of tumor cells to the target organ endothelium.
Localized mainly to the endoplasmic reticulum membrane, MTDH also translocates from the cytoplasm to the nucleus when induced by TNFalpha.
MTDH also localizes to tight junctions (TJ) during the maturation of TJ complexes in epithelial cells.
MTDH is found at highest levels in heart, skeletal muscle, tongue and small intestine.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of MTDH. Species Crossreactivity: Human, mouse, rat
