Contrin (MSy2 in mouse) and yB-2 (MSy3/4 in mouse) belong to the y-box family of multifunctional proteins that regulate both transcription and translation.y-box proteins interact with a wide variety of nucleic acid structures to act as transcription factors and mRNA masking proteins.
The modular structure of y-box proteins includes a highly conserved N-terminal cold-shock domain (CSD, equivalent to the bacterial cold-shock proteins) and four basic C-terminal domains containing arginine clusters and aromatic residues.
MSy2 (Contrin) is a expressed in testis and ovary, where it may repress translation of parental mRNA.
The gene encoding human Contrin maps to chromosome 17p11.2-13.1.
MSy3/4 (yB-2) is also known as DNA binding protein A and is highly expressed in testis, heart and muscle.
Contrin and yB-2 bind to the consensus sequence 5'-UCCAUCA-3' contained in the y-box element .
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MSY2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 287-325 of Human MSY2.
