Protein-bound methionine residues are among the most susceptible to oxidative stress by biological reactive oxygen species (ROS) such as H2O2, hydroxyl radicals and superoxide ions, which result in the formation of methionine sulfoxide, or Met(O).
Methionine sulfoxide reductase (MsrA) is an enzyme that catalyzes the thioredoxin-dependent reduction of Met(O) residues in proteins and in methyl sulfoxide compounds.
MsrA is a ubiquitously expressed protein which is found in organisms from yeast to man.
Mammalian MsrA is most highly expressed in liver, kidney, macrophages, neutrophils, cerebellum and brain neurons.
Oxidation of proteins by ROS is associated with oxidative stress and age-related diseases such as Alzheimer’s disease.
Recombinant mammalian MsrA retains enzymatic activity, and overexpression of the protein in yeast and human T cells increases their resistance to oxidative stress.
Furthermore, MsrA activity decreases in all regions of the Alzheimer’s disease brain.
These findings indicate that MsrA plays an important role in protecting cells against oxidative damage and early cell death.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of MSRA. Species Crossreactivity: Human
