84-2365-69　［取扱停止］MSH2 (DNA Mismatch Repair Protein Msh2, hMSH2, MutS Protein Homolog 2, MSH2) 100ul　224291

特徴

• Component of the post-replicative DNA mismatch repair system (MMR).
• Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair.
• When bound, heterodimers bend the DNA helix and shields ~20 base pairs.
• MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA.
• MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long.
• After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis.
• ATP binding and hydrolysis play a pivotal role in mismatch repair functions.
• The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone.
• This transition is crucial for mismatch repair.
• MutS alpha may also play a role in DNA homologous recombination repair.
• In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.