84-2365-50　［取扱停止］MRP2 (Canalicular Multispecific Organic Anion Transporter 1, ATP-Binding Cassette sub-Family C Member 2, Canalicular multidrug Resistance Protein, Multidrug Resistance-Associated Protein 2, ABCC2, CMOAT, CMOAT1, CMRP) 50ul　224282

特徴

• The two members of the large family of ABC transporters known to confer multidrug resistance in human cancer cells are the Mdr-1 P-glycoprotein and the multidrug-resistance protein MRP1.
• MRP1 is an integral membrane protein that contains an MDR-like core, an N-terminal membrane-bound region and a cytoplasmic linker, and it is expressed in various cerebral cells, as well as in lung, testis and peripheral blood.
• The MRP gene family also includes MRP2, which is alternatively designated cMOAT (for canalicular multispecific organic anion transporter), and MRP3, which are both conjugate export pumps ex-pressed predominantly in hepatocytes.
• MRP2 localizes exclusively to the apical membrane and is constitutively expressed at a high level in normal liver cells.
• Conversely, MRP3 localizes to the basolateral membrane where it also mediates the transport of the organic anion S-(2,4-dinitrophenyl-) glutathione toward the basolateral side of the membrane.
• MRP3 is normally expressed at comparatively lower levels than MRP2 and increases only when secretion across the apical membrane by MRP2 is impaired.
• MRP6 is highly expressed in liver and kidney, whereas MRP4 and MRP5 are detected in various tissues, yet at much lower levels of expression.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.