MRPL12 (mitochondrial ribosomal protein L12), also referred to as 5c5-2, L12mt, MRPL7 or RPML12, is a mammalian mitochondrial ribosomal protein that is involved in protein synthesis within the mitochondrion.
MRPL12 is enhanced in growth-stimulated cells as a result of transcriptional activation, suggesting that it may function as a translational regulator of mitochondrial mRNAs.
Impairment of MRPL12 leads to reduction in cell growth rate, decreased mitochondrial ATP production and abolition of mitochondrial oxidative phosphorylation.
MRPL12 is cleaved during its translocation across the mitochondrial membrane and it exists as dimers that bind the large ribosomal subunit.
MRPL12 is 198 amino acids in length, belongs to the ribosomal protein L12P family and is highly expressed in the colon.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MRP-L12. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 75-120 of Human MRP-L12.
