84-2365-21　［取扱停止］MRE11 (Double-strand break Repair Protein MRE11A, Meiotic recombination 11 Homolog 1, MRE11 Homolog 1, Meiotic recombination 11 Homolog A, MRE11 Homolog A, MRE11A, HNGS1, MRE11) 100ul　224266

特徴

• DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway.
• The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes.
• Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms.
• Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance.
• Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved.
• Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule.
• RAD54B is significantly homologous to the RAD54 recombination gene.
• Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination..
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.