Protein kinases comprise a large group of encoded factors that regulate cellular processes by catalyzing the transfer of a phosphate group to a hydroxyl acceptor in serine, threonine or tyrosine residues.
Myotonic dystrophy kinaserelated Cdc42-binding (DMPK-like) kinases-alpha and beta (MRCK-alpha, beta) contain a cysteine-rich motif and a putative pleckstrin homology domain.
MRCKs can phosphorylate nonmuscle Myosin light chain and influences Actin-Myosin contractility.
MRCK-alpha can phosphorylate and activate LIM kinases downstream of Cdc42, which leads to inactivation of ADF/Cofilin and to Actin cytoskeletal reorganization.
MRCK-alpha can also influence neurite outgrowth promoted by Cdc42 and Rac.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MRCKbeta. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1630-1680 of Human MRCKβ.
