84-2365-08　［取扱停止］mPRalpha ( Membrane progestin Receptor alpha, mPR alpha, Progestin and adipoQ Receptor Family Member 7, Progestin and adipoQ Receptor Family Member VII, MRPA, PAQR7) 50ul　224260

特徴

• The steroid progesterone induces the resumption of maturation in oocytes via a nongenomic pathway through binding to a novel, membrane progestin receptor (mPR).
• This pathway inhibits adenylyl cyclase and reduces intracellular cAMP, and also activates mitogen-activated protein kinase to effect signal transduction pathways.
• Three distinct groups, designated alpha, beta and y, comprise this gene family.
• mPRalpha, also designated progestin and adipoQ receptor family member VII (PAQR7), consists of an extracellular N-terminus, an intracellular C-terminus, and seven transmembrane domains.
• It is expressed in ovary, testis, placenta, uterus and bladder.
• mPRbeta, also designated progestin and adipoQ receptor family member VIII (PAQR8), consists of eight putative transmembrane regions and an intracellular N-terminus that contains a leucine-rich motif.
• It is a 354 amino acid protein with a molecular mass of about 40kDa and is expressed in brain and spinal cord.
• Both mPRalpha and mPRbeta may be G protein-coupled receptors and may be involved in oocyte maturation.
• Applications:Suitable for use in Immunofluorescence, Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.