Progression of cells from interphase to mitosis involves alterations in cell structures and activities.
The transition from G2 to M phase is induced by M phase-promoting factor (MPF).
In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF.
These M phase phosphoproteins (MPPs), also known as MPHOSPHs, permit disassembly of interphase structures and generation of M phase enzymatic activities and structures.
MPP9 (M-phase phosphoprotein 9), also known as MPHOSPH9, is a 1,031 amino acid peripheral membrane protein of the Golgi apparatus that exists as two alternatively spliced isoforms.
The gene encoding MPP9 maps to human chromosome 12, which encodes over 1,100 genes and comprises ~4.5% of the human genome.
Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MPP9. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 81-126 of Human MPP9.
