Progression through the cell cycle requires activation of a series of enzymes designated cyclin dependent kinases (Cdks).
The monomeric catalytic subunit, Cdk2, a critical enzyme for initiation of cell cycle progression, is completely inactive.
Partial activation is achieved by the binding of regulatory cyclins such as cyclin D1, while full activation requires phosphorylation at Thr 160.
The enzyme responsible for phosphorylation of Thr 160 in Cdk2 and also Thr 161 in Cdc2 p34, designated Cdk-activating kinase (CAK), has been partially purified and shown to be comprised of a catalytic subunit, a regulatory subunit and a subunit of unknown function.
The regulatory subunit is a novel cyclin (cyclin H) and is required for activation of Cdk7.
This previously undescribed protein, now termed Mat1, has been cloned as a protein that associates with the cyclin H-Cdk7 nuclear complex at all stages of the cell cycle.
Cyclin H-Cdk7-Mat1 complexes display kinase activity towards Cdk activation domains, and the carboxy terminus of RNA polymerase II.
Mat1 appears to constitute the first example of an assembly factor, essential for the formation of an active Cdk-cyclin complex.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of MNAT1. Species Crossreactivity: Human, mouse, rat
