Proteins of the matrix metalloproteinase (MMP) family are zinc containing proteolytic enzymes involved in the breakdown of the extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling.
They also play an important role in apoptosis, tumor cell growth, invasion, metastasis, as well as in angiogenesis and wound healing.
Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
Most of the MMP's contain a common domain structure, which include a signal sequence, a propeptide, a catalytic domain and a hemopexin-like (Hpx) domain.
MMP10 degrades proteoglycans, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V.
and fibronectin.
It activates procollagenase.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MMP-10. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 351-400 of Human MMP-10.
