As a result of the binding of growth factors to their membrane receptors, cytoplasmic proteins containing Src homology 2 (SH2) domains associate with specific phosphotyrosine residues within the activated receptors and function as signaling intermediates.
The action of such SH2 domain proteins frequently involves the activation of a second group of signaling intermediates characterized by SH3 domains.
These latter proteins function through binding proline-rich sequences in target proteins.
A novel human non-receptor protein kinase, designated either MLK3 or PTK1, is 847 amino acids in length and contains an SH3 domain in the absence of an SH2 domain.
In addition, MLK3 is characterized by a leucine zipper basic region (a motif commonly associated with transcription factors) and has a long carboxy-terminal tail which exhibits proline-rich motifs similar to known SH3 binding sites.
MLK3 is expressed widely and is related to the previously described MLK1 and MLK2 kinases.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes endogenous levels of human MLK3, phosphorylated T277/S281. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human MLK3 around the phosphorylation site of Threonine 277 and Serine281.
