84-2363-45　［取扱停止］MGMT (6 O methylguanine DNA Methyltransferase, Agat, AGT, AI267024, EC 2.1.1.63, Methylated DNA Protein cysteine Methyltransferase, Methylguanine DNA Methyltransferase, MGC107020, MGMT, MGMT: O-6 methylguanine DNA Methyltransferase, O 6 methylguanine DNA alkyltransferase, O 6 methylguanine DNA Methyltransferase, O-6 methylguanine DNA alkyltransferase, O-6-methylgu ...　224176

特徴

• MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs.
• MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53.
• Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation only in cell lines that express functional p53, and not in cell lines that do not express wild type p53.
• In contrast, in a study of oral cancer cell lines, high MGMT activity was associated with the presence of mutant p53.
• Similarly, MGMT activity was significantly lower in ovarian tumors with wild-type p53 than in tumors with mutant p53, supporting the view that wild type p53 down-regulates the basal MGMT promoter.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.