84-2361-99　［取扱停止］MEF2D, phosphorylated (S444) (MEF2D,Myocyte-specific Enhancer Factor 2D) 100ul　224103

特徴

• The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells.
• Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C, and -2D.
• These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes.
• Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development.
• The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression.
• The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors.
• A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family.
• Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:150Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.