Med30 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex.
The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes.
The mediator complex functions as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, and is recruited to promoter regions by directly interacting with regulatory proteins.
The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors.
Found in the nucleus, Med30 is expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity:Recognizes endogenous levels of MED30. Species Crossreactivity: Human, mouse, rat
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:A synthetic peptide corresponding to residues in Human MED30.