Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex.
The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes.
Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins.
The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors.
Med22 (Mediator complex subunit 22), also known as SURF5 (Surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex.
There are two isoforms of Med22 that are produced as a result of alternative splicing events.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MED22. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human MED22.
