The mini-chromosome maintenance (MCM) family of proteins include MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5), MCM7 (Cdc47), MCM8, MCM9 and MCM10 (Dna43).
The proteins in this family are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle.
Expression of MCM proteins increases during cell growth, peaking at G1 to S phase.
MCM proteins are crucial components of the pre-replication complex (pre-RC) and are involved in replication fork formation and the recruitment of other DNA replication proteins.
The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP- dependent opening of double-stranded DNA.
MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell.
Throughout the cell cycle, MCM8 binds chromatin.
MCM8 can interact with CDC6 and ORC2L.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MCM8. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human MCM8.
