B cell CLL/lymphoma-2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal.
Myeloid cell leukemia-1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability.
p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity.
Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors.
Glycogen synthase kinase-3 (GSK-3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors PI 3-kinase and Akt.
Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of human Mcl-1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 100-150 of human Mcl-1.
