Melanin-concentrating hormone (MCH) is a 19 amino acid cyclic neuropeptide,which is mainly expressed in the hypothalamus.
MCH modulates feeding behavior, aggression, anxiety, arousal and reproductive function in mammals by controlling the release of luteinizing hormone (LH).
The melanin-concentrating hormone receptor (MCHR, also designated SLC-1) is a glycosylated G-protein coupled receptor that migrates between 45-50kDa on SDS-PAGE.
MCHR mediates the effects of MCH through Gai and/or Gaq signaling and is expressed in several regions of the brain, including the cerebral cortex, amygdala, thalamus and hypothalamus.
MCH and MCHR have also been implicated in stimulating leptin expression and secretion in adipocytes, which suggests that the melanin-concentrating hormone and its receptor may be potential targets for modulating obesity.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MCH-2R. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 300-348 of Human MCH-2R.
