The tumorigenic and metastatic phenotype of melanoma cells correlates well with an increased expression of cell-cell and cell-matrix adhesion receptors.
The human Mel-CAM gene encodes a transmembrane glycoprotein, also designated MCAM, MUC18 or CD146, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule.
The deduced human sequence of 603 amino acids consists of a signal peptide, five immunoglobulin-like domains, a transmembrane region and a short cytoplasmic tail.
Mel-CAM expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes.
Mel-CAM is found on 80% of advanced primary human mela-nomas and correlates well with development of metastatic disease.
Mel-CAM activation initiates an outside-in signaling pathway that involves the protein tyrosine kinases Fyn, FAK and paxillin.
Mel-CAM influences the dynamics of Actin cytoskeleton rearrangement and is essential for the maintenance of thymic architecture and function.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human MCAM. Species Crossreactivity: mouse, rat
