MC4 receptor is the fourth member of the five known melanocortin receptors (MCRs) belonging to the rhodopsin-like family of G protein-coupled receptors (GPCRs).
These five MCRs form a unique family of GPCRs, marked by their unusually short coding sequence and the absence of highly conserved amino acid residues or motifs common to most GPCRs.
All MCRs signal through the cyclic adenosine 3', 5'-monophosphate (cAMP) pathway.
With the exception of MC2R, all MCRs are activated by both adrenocorticotropin (ACTH) and melanocyte-stimulating hormones (MSHs and mainly alpha-MSH).
The MC4R receptor is expressed primarily in the brain, and unlike the other melanocortin receptors, MC4R is not expressed in adrenal cortex or in melanocytes.
Genetic studies of mice and humans have established a critical role of MC4 Receptor in appetite regulation.
Heterozygous mutations in MC4 Receptor account for 1-6% of severe cases of human obesity.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MC4-R. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 300-350 of Human MC4-R.
