It is now well established that the nature and relative abundance of individual subunits of different classes of transcription factors can positively or negatively regulate levels of gene expression.
Myc proteins homodimerize and bind DNA poorly, if at all, at physiological levels.
Max is a nuclear localized bHLH-Zip protein initially identified by screening a B cell expression library with the bHLH-Zip region of c-Myc.
Max homodimers and the Myc-Max heterodimers bind the sequence CACGTG; however the binding of the hetero-dimeric complex is stronger than the Max homodimer.
The Max gene products have been identified as Max and Max 9 proteins that differ by a 9 amino acid insertion N-terminal to the basic region.
In contrast to Myc, which is highly regulated during progression through the cell cycle, Max is highly stable and is much more abundant than Myc.
Two members of the bHLH-Zip protein family, designated Mad and Mxi1, homodimerize poorly but form heterodimeric complexes with Max that have opposing functions to Myc-Max heterodimers with respect to regulation of gene expression.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MAX. Species Crossreactivity: Human, mouse, rat
