Maspin is structurally a serine protease inhibitor (serpin) that was initially isolated from normal human mammary epithelial cells.
Serpins are a family of proteins that inhibit chymotrypsin-like serine proteinases.
Serpins control activated proteinases and several are involved in the regulation of cell death.
Maspin is found in the extracellular matrix and at the plasma membrane.
Maspin has been shown to act at the cell surface to block cell motility and inhibit invasion of breast and prostate cancer cells.
Maspin is present in normal mammary epithelial cells but is absent in many tumor cell lines, yet no major structural alterations of the Maspin gene have been identified in tumor cells.
Similarly, Maspin is expressed in normal prostate cells and downregulated or absent in prostate tumor cells..
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Maspin. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 100-150 of Human Maspin.
