Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2).
Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3).
Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3) (4), and subsequently found to contain similarity to the yeast protein Apg8/Aut7/Cvt5 critical for autophagy (5).
Three human LC3 isoforms (LC3A, LC3B, and LC3C) undergo post-translational modifications during autophagy (6-9).
Cleavage of LC3 at the carboxy terminus immediately following synthesis yields the cytosolic LC3-I form.
During autophagy, LC3-I is converted to LC3-II through lipidation by a ubiquitin-like system involving Atg7 and Atg3 that allows for LC3 to become associated with autophagic vesicles (6-10).
The presence of LC3 in autophagosomes and the conversion of LC3 to the lower migrating form LC3-II have been used as indicators of autophagy (11).
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of MAP1LC3A. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human MAP1LC3A.
Shelf Life：1year
Swiss Prot Number：Q9H492
EU Commodity Code：30021010
商品名：MAP1LC3A (ATG8E, Autophagy-Related Protein LC3 A, Autophagy-Related Ubiquitin-like modifier LC3 A, LC3, LC3A, MAP1 Light Chain 3 like Protein 1, MAP1 Light Chain 3-like Protein 1, MAP1A/1B Light Chain 3 A, MAP1A/MAP1B LC3 A, MAP1A/MAP1B Light Chain 3 A, MAP1ALC3, MAP1BLC3, Map1lc3a, Microtubule Associated Proteins 1A/1B Light Chain 3, Microtubule-Associated Protein 1 Light Chain 3 alpha, Microtubule-Associated Proteins 1A/1B Light Chain 3A, MLP3A_HUMAN,) 50ul
