MAGE-C2/C3 (Melanoma-associated antigen C2, Hepatocellular carcinoma-associated antigen 587) is a 373 amino acid protein encoded by the human gene MAGEC2.
Genes of the MAGE family direct the expression of tumor antigens that are recognized on human melanomas by autologous cytolytic T lymphocytes.
Among tumor tissues, MAGE-C2/C3 is frequently expressed in seminomas, melanomas, and bladder transitional cell carcinomas.
It is also expressed in a significant fraction of head and neck carcinomas, breast carcinomas, nonsmall-cell lung carcinomas, and sarcomas.
In hepatocellular carcinoma, there is a inverse correlation between tumor differentiation and protein expression, i.e.
the lower the differentiation, the higher percentage of expression.
MAGE-C2/C3 is not expressed in normal tissues, except in germ cells in the seminiferous tubules and in Purkinje cells of the cerebellum.
It is strongly expressed in spermatogonia and primary spermatocytes, however, at later stages of maturation, expression gradually decreases to become undetectable in mature spermatids.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of MAGEC2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 237-282 of Human MAGEC2.
