M-RIP (myosin phosphatase Rho interacting protein), also known as MPRIP, p116Rip, RIP3 or RHOIP3, is a 1,025 amino acid cytoplasmic and cytoskeletal protein that is required for regulation of the actin cytoskeleton.
M-RIP colocalizes with myosin binding subunit (MBS) to regulate the phosphorylation of myosin light chain, and colocalizes with F-actin through its N-terminus in the cytoskeleton.
M-RIP also interacts with and RhoA at actin stress fibers via its adjacent coiled coil domains.
M-RIP is highly expressed in ovary, with moderate levels found in brain, heart, liver, lung, skeletal muscle, testis and kidney.
M-RIP depletion causes an increase of stress fibers in smooth muscle cells, whereas M-RIP over-expression causes disassembly of stress fibers in neuronal cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of M-RIP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 756-801 of Human M-RIP.
