The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis.
A group of proteins that are intimately involved in this process are the serine/ threonine (Ser/Thr) protein kinases.
LOK (lymphocyte-oriented kinase), also known as STK10 (serine/threonine kinase 10), is a 968 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase family.
Expressed in lymphoid organs, LOK functions to catalyze the ATP-dependent phosphorylation of target proteins, such as MBP (myelin basic protein) and Histone H2A, thereby playing a role in signaling pathways throughout the cell.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of LOK. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 501-550 of Human LOK.
