The LIM-only (LMO) proteins, LMO1 and LMO2, are nuclear factors that are characterized by a conserved LIM domain.
The LIM domain consists of a cysteine-rich zinc-binding motif that is present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system and involved in cell differentiation.
LMO1 and LMO2 are expressed in the adult CNS in a cell type-specific manner, where they are differentially regulated by neuronal activity and are involved in regulating the cellular differentiated phenotype of neurons.
LMO2 lacks a specific DNA-binding homeobox domain but rather assembles into transcriptional regulatory complexes to mediate gene expression by interacting with the widely expressed nuclear LIM interactor (NLI).
NLI, known also as CLIM-1, and the related protein CLIM-2 facilitate the formation of heteromeric LIM complexes and also enhance the nuclear retention of LIM proteins.
LMO2 and the related protein LMO4 are expressed in thymic precursor cells.
LMO4 is also expressed in mature T cells, cranial neural crest cells, somite, dorsal limb bud mesenchyme, motor neurons, and Schwann cell progenitors.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of LMO2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human LMO2.
Shelf Life：1year
Swiss Prot Number：P25791
EU Commodity Code：30021010
商品名：LMO2 (Cysteine Rich Protein TTG 2, Cysteine Rich Protein TTG2, Cysteine-Rich Protein TTG-2, LIM Domain only 2 (rhombotin like 1), LIM Domain only 2, LIM Domain only Protein 2, LMO 2, LMO-2, lmo2, RBTN 2, RBTN L1, RBTN2, RBTN2_HUMAN, RBTNL 1, RBTNL1, RHOM 2, RHOM2, Rhombotin 2, Rhombotin like 1, Rhombotin-2, Rhombotin2, T Cell Translocation Gene 2, T Cell Translocation Protein 2, T-Cell Translocation Protein 2, TTG 2, TTG2) 50ul
