The Lipase gene family is part of one of the largest genetic superfamilies in living organisms.
Members of the AB hydrolase subfamily all contain an enzyme core with an alpha/beta sheet, not barrel, of eight beta-sheets connected by alpha-helices.
The AB hydrolase subfamily plays a crucial role in the metabolismof lipids.
Members of this family include hepatic lipase (HL), endothelial lipase(EL), lipoprotein lipase (LPL), pancreatic lipase (PL), gastric lipase (GL), LCAT, and lysosomal acid lipase (LAL).
LAL is the important enzyme in the hydrolysis of triglycerides and cholesteryl esters in lysosomes.
LAL has six potential N-glycosylation sites and one potential O-glycosylation site, and it is mediated by macrophage mannose receptors.
Defects in the LAL gene have been linked to Wolman disease (WD) and CE storage disease (CESD), while overexpressionof LAL leads to atherosclerosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of LIPA. Species Crossreactivity: Human
