84-2358-06　［取扱停止］LIG3 (DNA Ligase 3, DNA Ligase III, Polydeoxyribonucleotide Synthase [ATP] 3, LIG3) 100ul　223903

特徴

• DNA ligase is a type of ligase that can link together DNA strands that have double-strand breaks.
• DNA ligase functions in both DNA repair and DNA replication.
• It is utilized in molecular biology laboratories for recombination experiments.
• In mammals, the four specific types of DNA ligase are known as DNA Ligase I, II, III and IV.
• DNA Ligase I ligates Okazaki fragments during lagging strand DNA replication and some recombinant fragments.
• DNA Ligase II is an alternatively spliced form of DNA Ligase III found in non-dividing cells.
• DNA ligase III complexes with the DNA repair protein XRCC1 to function in sealing base excision mutations and recombinant fragments.
• DNA Ligase IV complexes with XRCC4 and catalyzes the final step in the non-homologous end joining DNA double-strand break repair pathway.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.