As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10.
Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN (also designated MMAC1 and TEP1), DMBT1 (for deleted in malignant brain tumors 1) and LGI1 (for leucine-rich gene-glioma inactivated 1) candidate tumor suppressor genes.
The PTEN gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers.
Reduced levels of DMBT1 mRNA have been noted in gastrointestinal and esophageal cancers as well as in gliomas.
LGI1, which is highly specific for neural tissues, shares homology with several transmembrane and extracellular proteins that function as receptors and adhesion proteins.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of LGI1. Species Crossreactivity: Human, mouse, rat
