The Laminins comprise a growing family of disulfide-linked heterotrimers consisting of three genetically distinct polypeptide chains, designated alpha, beta and y.
A major component of the basal lamina, Laminins play a crucial role in providing a scaffolding upon which tissues are assembled and which serves as a physical barrier separating specialized tissues.
During embryogenesis and early development, cells migrate along basement membranes, which are required for the polarization of cells.
The beta-2 laminin chain plays a role in melanoma spread, promoting tumor migration along the abluminal surface of vessel, a phenomenon which has been termed extra-vascular migratory metastasis.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of human Laminin beta. Species Crossreactivity: mouse, and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 80-130 of human Laminin β-2.
