Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes.
Basement membranes (cellassociated extracellular matrices (ECMs)) are polymers of laminins with stabilizing type IV collagen networks, nidogen, and several proteoglycans.Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells.
Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture.
Each laminin is a heterotrimer of alpha, beta, and y chain subunits that undergoes cell-secretion and incorporation into the ECM.
Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors.
The human laminin alpha-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Laminin alpha. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 480-530 of Human Laminin α-4.
